JEOL Automated Data Acquisition System (JADAS) software was developed in collaboration with NCMI Baylor College of Medicine for cryoelectron microscopy. Using JADAS, researchers can customize parameters for ice-embedded macromolecular complexes under low dose conditions. The software acquires large quantities of high quality data without operator intervention. The sample stage moves sequentially to create a complete grid atlas. The software evaluates ice thickness, defocus and drift, among other parameters, before acquiring the high resolution data.