Analytical Instrument Documents

It has been a decade since Dodonov and his colleagues first announced the electrospray ionization time-of-flight mass spectrometer (ESI-TOF MS). Their initial findings have been enhanced by Standing and others, and it has been recently reported that a large TOF MS system achieved a mass resolution exceeding 10,000.

Mass spectrometers are commonly combined with separation devices such as gas chromatographs (GC) and liquid chromatographs (LC). The GC or LC separates the components in a mixture, and the components are introduced, one by one, into the mass spectrometer. MS/MS is an analogous technique where the first-stage separation device is another mass spectrometer.

Accurate mass measurements are frequently used to determine the elemental composition for molecular and fragment ions. These measurements are normally obtained by acquiring a high resolution mass spectrum so that the masses are measured for a single species and not measured for unresolved or partially resolved peaks.

What does a mass spectrometer do? What are mass spectrometers used for? What does the mass tell us? How does a mass spectrometer work?

Several different definitions of resolution are used in mass spectrometry. It is useful to understand the distinctions between the different definitions to understand the characteristics of different mass spectrometers.

The JMS-S3000 "SpiralTOF™" is a MALDI-TOFMS that incorporates an innovative SpiralTOF ion optics system. The JMS-S3000 is available with a TOF-TOF option that can acquire high-energy collision-induced dissociation (CID) product ion spectra for monoisotopically selected precursor ions.

High molecular weight polymers are often MS-silent due to their inherent high dispersity (DM) or detected in the high mass range with low resolving power. An "on-plate" alkaline degradation has thus been developed as a sample pre-treatment on the MALDI target with tenths of ng of polymer to cut long industrial polyester chains into short oligomers amenable to MALDI-HRMS.

The JMS-S3000 "SpiralTOF™" is a MALDI-TOF MS incorporating an innovative SpiralTOF™ ion optics system. JEOL's patented technology achieves a spiral ion trajectory of 17m within a compact 1m space.

The JMS-S3000 SpiralTOF has a unique 17m flight path that offers then highest resolution MALDI-TOF MS system currently available. However, ions with a very short lifetime or that undergo spontaneous dissociation during their flight cannot be detected by the SpiralTOF (or a conventional reflection TOF). To address this situation, the SpiralTOF with Linear TOF option can be used for the high sensitivity analysis of intact proteins.

Diesel Fuel Analysis by GCxGC/EI (Hydrocarbon Classification); Biomarker Analysis by GCxGC/PI (Target Analysis using 2D EICC); Type Analysis by GCxGC/FI (Hydrocarbon Type Analysis); Synthetic Polymer Analysis by Pryolysis GCxGC/EI and FI (Unknown Compounds Analysis in Nylon 66); Additives Analysis by Pyrolysis GCxGC/EI and FI (Targeted Additives Analysis in Nitrile Butadiene Rubber); Natural Polymer Analysis by Pyrolysis GCxGC/EI and PI (Powerful Separation of the Main Components in a Japanese Lacquer Film); Soluble Organic Fraction Analysis by GCxGC/EI (Analysis of PAHs in Exhaust Gas); Electronic Waste Analysis by GCxGC/EI and Negative CI (Halogenated Compounds Analysis); Aroma Oil (Fragrance) Analysis by GCxGC/EI and FI (Molecular ion detection for Alcohol compounds); Sebum Analysis by GCxGC/EI (Pharmaceutical application)