Convert AccuTOF™ LC into Nano-LC/MS System: An Application of Peptide Analysis
Introduction
Nano-LC/MS has demonstrated many advantages, including lower sample consumption, higher mass sensitivity and less matrix effect. Here, we introduce a very simple method to convert regular AccuTOF™ LC system into a nano-LC/MS system without high cost. An application of peptide analysis was used as an example to test the nano-LC/MS system. The RSD of retention time for gradient elution is less than 1%.
What you need
- AccuTOF time-of-flight mass spectrometer (JEOL)
- Agilent 1100 HPLC with binary pump (JEOL or Agilent)
- NanoESI source (JEOL)
- 6 port external loop sample injector (Valco, part number C2-0006)
- Peek tee (Upchurch, part number P-727)
- Peek tubing, 1/16’’ OD x .0025" ID (Upchurch, part number 1560)
- Microtight sleeve, .0155 x .025 (Upchurch, part number F-185X)
- Microtight union (Upchurch, part number P-720)
- SilicaTip™ Emitter
- (New Objective, part number FS360-50-30-CE-5-C15 for the flow rate of 300 – 1000 nL/min;
part number FS360-75-15-CE-5-C15 for the flow rate of 200 – 500 nL/min)
- Integrafrit column (recommend New Objective Proteopep II™ C18 column, part number IFC75-PP2-10)