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Simultaneous determination of residual agricultural chemicals in food by GC-MS/MS - MSTips259

Product: JMS-TQ4000GC GC/MS/MS System

Introduction

As "food safety" is recognized as an increasingly important issue on a global scale, many nations have their own regulations on residual agricultural chemicals in food. In Japan, the positive list system, which was enforced at the end of May 2006, stipulates a uniform standard of 10 ppb as a quantity that is considered safe for human health. Under the positive list system, more agricultural chemicals need to be examined, and as a result, techniques capable of accurately and collectively analyzing residual agricultural chemicals in food are in increasing demand. While mass spectrometry (MS) is known for its high detection sensitivity, MS/MS is becoming the mainstream of pesticide analysis for its superior sensitivity and selectivity. The JMS-TQ4000GC, JEOL’s latest GC-MS/MS system, has a unique ion storage/ejection mechanism within the MS/MS collision cell and incorporates new firmware to support MS/MS analysis with up to 36,000 transitions. In this work, we report the verified results for pesticide residues sensitivity in food using the JMS-TQ4000GC.

Experiment

A pesticide standard solution from FUJIFILM Wako Pure Chemical Corporation (PL series) was used that consisted of equal amounts of PL 1, 2, 3, 4, 5, 6, 11, and 12. Afterwards, the solution was diluted to 1, 5, 10, 50 and 100 ppb. PEG 300 was used to protect the pesticides from thermal decomposition in the GC injection liner. In this work, both the sensitivity and the linearity of the calibration curve were examined for 150 pesticides. Table 1 shows the measurement conditions used for the analysis.

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